Deoxyribonuclease I instructions in Chinese and English

Enzymatic Reaction： 

Bovine pancreatic deoxyribonuclease is an endonuclease that preferentially splits phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5’-phosphate terminated polynucleotides with a free hydroxyl group at the 3’ position. DNase I is secreted by exocrine glands, and found most abundantly in the pancreas and parotid. It is also present in lower quantities in other tissues (Chen and Liao 2006, and Nadano et al. 1993).

DNase is known to be involved in apoptosis and has been proposed to play a role in the regulation of actin polymerization in cells. In addition to its use in molecular biology, DNase I has been used as a treatment for cystic fibrosis, and systemic lupus erythematosus (Chen and Liao 2006).

Specificity:

bpDNase I is not base nor sequence specific; however, it does not cleave randomly. It shows preference for cleavage at the 5’ side of pyrimidines, and is particularly pronounced in alternative copolymers (Bernardi et al. 1975, and Lomonossoff et al. 1981). It has been shown that variations in the twist angle are recognized by DNase I (Dickerson and Drew 1981). The specificity of DNase I also depends on the divalent cations present. In the presence of Ca2+ and Mg2+, it causes single strand breaks, and in the presence of Mn2+ double strand breaks have been reported (Junowicz and Spencer 1973, and Campbell and Jackson 1980).

Applications:

DNA removal in primary cell isolation: decreases viscosity providing better yields

DNA removal in bioprocessing applications

Removing genomic DNA from RNA preparations prior to RT-PCR

in vitro transcription

Nick translation

DNase footprinting

Actin binding

UV crosslinking of proteins to nucleic acids

Radioactive labeling

AI Meijie Worthington Deoxyribonuclease I Specificity ：

bpDNase I  Not base or sequence specific ; however , It does not cut randomly . It shows priority in pyrimidine  5'  Side cracking , And especially in alternative copolymers （Bernardi  wait forsomeone  1975  and  Lomonossoff  wait forsomeone  1981）. It has been shown that , The change of twist angle is  DNase I  distinguish （Dickerson  and  Drew 1981）.DNase I  The specificity also depends on the presence of divalent cations . In existence  Ca2+  and  Mg2+  Under the circumstances , It can cause single chain breaks , But in being  Mn2+  It will cause double chain breakage （Junowicz  and  Spencer 1973, as well as  Campbell  and  Jackson 1980）.

AI Meijie Worthington Deoxyribonuclease I Molecular characteristics ：

And human 、 Mice are similar to rats , Bovine pancreas  DNase I  Genes are made up of  9  Composed of exons , Only the last  8  Exons encode the protein （De María  and  Arruti 2003）. The nascent protein is encoded by exon 2  22  Amino acid signal sequences are directed to secretory pathway organelles . Main active site residues  H166  By exons  6  code （De María  and  Arruti 2003  and  Kraehenbuhl  wait forsomeone  1977）. Although the exons of cattle and other mammals are almost the same length , But the length of introns varies greatly .TATA  Box sequence in exon  I  The upstream  35 bp（De María  and  Arruti 2003）. It has been suggested that the multiple splicing events that affect the coding sequence may be down-regulation  DNase I  The mechanism of expression （Liu  wait forsomeone  1997）.

Deoxyribonuclease I Related research ：

Actin

Albumin , Nuclease free

Deoxyribonuclease  II

Deoxyribonucleic acid and related products

Histone

Lysozyme

Nuclease , Micrococcus

Nuclease ,S1

Phosphatase , alkalinity

Phosphodiesterase  I

Phosphodiesterase  II

protease K

Reverse transcriptase , restructuring  HIV

Ribonuclease A

Ribonuclease  T1

Ribonuclease ,E-Rase RNase  Mixture

RNA