Mbio | the sun Chaomin formation of Ocean Institute has verified in situ the new pathway of microbial mediated elemental sulfur formation in the deep sea

https://journals.asm.org/doi/10.1128/mbio.00143-22

7 month 20 Japan , International Academic Journal mBio Published the title “Deep-sea in situ insights into the formation of zero-valent sulfur driven by a bacterial thiosulfate oxidation pathway” The article , This paper reports the research results of the sun Chaomin research group of the Institute of Oceanography, Chinese Academy of Sciences, in verifying the new pathway of bacterial oxidation of sodium thiosulfate to form elemental sulfur in the deep-sea cold spring in situ environment , It provides a referential means for deep-sea in-situ study of microbial special life processes , It also provides theoretical evidence for explaining the cause of the widespread distribution of sulfur in the cold spring vents in the South China Sea . 

In the early scientific investigation , Based on Raman spectroscopy, the Zhang Xin research group of the Institute of Oceanography observed that the content of elemental sulfur in the cold spring environment of the South China Sea is rich , But the cause is not clear . Sun Chaomin's research team found a cold spring bacterium Erythrobacter sp. 21-3 It can efficiently oxidize sodium thiosulfate to form elemental sulfur based on a new sulfur oxidation pathway , This new sulfur oxidation pathway exists in many deep-sea microorganisms , It has an important contribution to the deep-sea sulfur cycle （ISME J, 2020）. But limited to various reasons , Whether this sulfur oxidation pathway occurs in situ in the deep sea has not been verified . With the help of 2020 year “ Science number ” The South China Sea Cold Spring voyage of the investigation ship , Sun Chaomin's research group will cold spring bacteria Erythrobacter sp. 21-3 And its mutant strain were cultured in situ near the deep-sea cold spring vent 10 God , After recovering the bacterial culture , Comprehensive use of Raman spectroscopy 、 Proteomics 、 Molecular biology and molecular genetics have confirmed that the strain can indeed form sulfur in situ in the deep sea , And knock out the key genes in the sulfur oxidation pathway tsdA(Thiosulfate dehydrogenase) And soxB (Thiosulfohydrolase) after , The ability of bacteria to form elemental sulfur has decreased significantly , This is highly consistent with the research results of the Laboratory . Different from the laboratory results tsdA Knockout strains cannot use thiosulfate to produce elemental sulfur under laboratory conditions , But in situ conditions can produce elemental sulfur , Through research, it is found that the mutant can use TETRATHIONATE widely existing in deep-sea cold springs to start the above sulfur oxidation pathway to form elemental sulfur ; Under laboratory conditions , Tetrasulfate requires TsdA Metabolic thiosulfate formation . The differences in the above conclusions also suggest that deep-sea researchers need to comprehensively consider the differences in conditions between laboratory and deep-sea in situ , Only through more comprehensive experimental verification can we truly understand the real life process of deep-sea microorganisms , The smooth progress of these studies requires Biology 、 geological 、 The interdisciplinary cooperation of physical ocean and other disciplines can be completed . , in turn, , The researchers found that compared with those that can produce a small amount of elemental sulfur tsdA Knockout strain , Does not produce elemental sulfur soxB The downregulated proteins of knockout strains are generally concentrated in the energy production and conversion pathways . Accordingly , Researchers have confirmed E. flavus 21-3 Elemental sulfur can be used as a nutrient source for growth both in the laboratory and in situ in the deep sea . by comparison , In situ ,E. flavus 21-3 The production pathway of elemental sulfur is important for its growth 、 It is more important for the generation and transformation of energy . Last , Based on metagenome sequencing, researchers found that the surface layer of cold spring sediment with the largest abundance of thiometagene , Bacterial genomes with newly discovered sulfur oxidation pathways account for about% of the total assembled genomes 25%, It is believed that this passage has certain universality in cold springs , It is speculated that it has an important contribution to the sulfur cycle of cold springs . 

Flow chart of deep-sea in situ experiment

Deep sea in situ verification of the process of bacteria producing elemental sulfur based on a new sulfur oxidation pathway  

Proteomics means to study the biological function of bacteria to produce elemental sulfur under laboratory and in situ conditions  

Macrogenomic analysis of sediments at different depths in deep-sea cold spring in situ experiment sites  

The first author of this research achievement is cairuining, a doctoral student of the Key Laboratory of Experimental Marine Biology , Researcher sun Chaomin is the corresponding author . The research was carried out by Qingdao Marine Science and technology pilot National Laboratory “ the 14th Five-Year Plan ” Major projects 、 Chinese Academy of Sciences strategic pilot project 、 The key deployment project of the center for Marine Science Research of the Chinese Academy of Sciences and other projects are jointly funded . 

Related papers ：Ruining Cai, Wanying He, Rui Liu, Jing Zhang, Xin Zhang, Chaomin Sun*. Deep-sea in situ insights into the formation of zero-valent sulfur driven by a bacterial thiosulfate oxidation pathway. mBio, 2022. Doi: 10.1128/mbio.00143-22.

Thesis link ：https://journals.asm.org/doi/10.1128/mbio.00143-22

Guess you like

iMeta brief introduction   Highly quoted articles   High color value drawing imageGP  Network analysis iNAP
iMeta Web tools   Metabolism group MetOrigin  Meggie cloud Lactation prediction DeepKla
iMeta review   Intestinal flora   Plant flora   Oral flora   Protein structure prediction

10000+： Flora analysis   Babies and dogs   Rhapsody of syphilis   carry DNA Hair Nature

Series of tutorials ： Introduction to microbiome  Biostar  Microbiome    Macro genome

Expertise ： Academic charts   High score article   Shengxin Scripture   An indispensable person

Article to read ： Macro genome   Parasite benefits   Evolutionary tree   Necessary skills ： put questions to   Search for   Endnote

Amplification analysis ： Chart interpretation   Analysis process   Statistical mapping

Biological science :   Intestinal bacteria   Life on the human body   Great leap forward in life    Cell warfare   The mystery of human body   

Written in the back

To encourage readers to communicate and quickly solve scientific research difficulties , We established “ Macro genome ” Discussion groups , There are already domestic and foreign 6000+ Researchers join . Please add editor in chief wechat meta-genomics Bring you into the group , Be sure to remark “ full name - Company - Research direction - The title / grade ”. Please indicate your identity for the senior title , There are also microorganisms at home and abroad PI Cooperation and exchange of group supply . Ask for help with technical problems , First read 《 How to ask questions gracefully 》 Learn how to solve problems , Unresolved intra group discussions , Don't talk about problems in private , Help colleagues .

Click to read the original text , Jump to the latest article catalog to read