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改性三磷酸盐研究:Lumiprobe氨基-11-ddUTP
2022-06-28 04:55:00 【Sylvia_sc】
艾美捷Lumiprobe Amino-11-ddUTP 是一种终止子三磷酸,用于 DNA 的酶末端标记。核苷酸通过 Taq DNA 聚合酶、Sequenase、Klenow 片段掺入。氨基末端 DNA 与活化酯的后续反应可用于 DNA 的末端标记。

艾美捷 Lumiprobe氨基-11-ddUTP化学性质:
形态: 无色固体
分子量:960.44
CAS号: 439077-17-5(不含阳离子和结晶水)
分子式:C 18 H 62 Li 3 N 4 O 32 P 3
溶解度:水中好
质量控制:NMR 1 H 和31 P, HPLC-MS (99+%)
储藏条件:储存:-20°C 24 个月。运输:在室温下长达 3 周。干燥。
艾美捷Lumiprobe 氨基-11-ddUTP相关文献:
1、Bakker, R.; Mani, M.; Carthew, R.W. The Wg and Dpp morphogens regulate gene expression by modulating the frequency of transcriptional bursts. bioRxiv, preprint. doi: 10.1101/2020.01.24.918623
2、Boersma, S.; Rabouw, H.H.; Bruurs, L.J.M.; Pavlovič, T.; van Vliet, A.L.W.; Beumer, J.; Clevers, H.; van Kuppeveld, F.J.M.; Tanenbaum, M.E. Translation and Replication Dynamics of Single RNA Viruses. Cell, 2020, 183(7), 1930–1945.e23. doi: 10.1016/j.cell.2020.10.019
3、Yu, Y.; Guo, Y.; Tian, Q.; Lan, Y.; Yeh, H.; Zhang, M.; Tasan, I.; Jain, S.; Zhao, H. An efficient gene knock-in strategy using 5'-modified double-stranded DNA donors with short homology arms. Nature Chemical Biology, 2020, 16(4), 387–390. doi: 10.1038/s41589-019-0432-1
Lumiprobe氨基-11-ddUTP相关研究:
氨基-11-dUTP
Amino-11-dUTP 是一种用于修饰 DNA 的三磷酸盐。
氨基-11-UTP
用氨基标记的三磷酸尿苷,用于间接非放射性酶促 RNA 标记。
生物素-11-dCTP
用于非放射性 DNA 标记的生物素化 dCTP。
生物素-11-dUTP
生物素标记的脱氧尿苷三磷酸,用于酶促掺入 DNA 和间接检测。
生物素-11-UTP
用于酶促 RNA 标记的生物素化 UTP。
FAM-11-dCTP,6-异构体
用于酶促 DNA 标记的 6-FAM 标记的 dCTP。
FAM-11-dUTP,6 异构体
用于 DNA 标记的具有绿色发射的 dUTP 的 FAM 衍生物。
FAM-11-UTP,6 异构体
6-FAM-三磷酸尿苷用于体外转录过程中的非放射性酶 RNA 标记。
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