Multi type study of Worthington collagen protease

Protease is widely found in animal viscera 、 Plant stem and leaf 、 In fruits and microorganisms . Microbial protease , Mainly by mold 、 bacteria , Then yeast 、 Actinomycete production . Collagenase , Also known as bone collagenase , Collagenase for short . By Clostridium histolyticum （Clostridum histolytirum） Made from .

Collagenase Of effect

1、 Collagenase Ⅰ For epithelium 、 lung , Separation of adipose and adrenal tissue cells . It is used to digest the connective part of the tissue to make it into a single thin layer Cell , For the separation of mammalian cells .

2、 Collagenase Ⅳ Include at least 7 A protease component , The molecular weight ranges from 68-130KD Unequal . It can digest many kinds of tissues .

3、 Collagenase Ⅴ Include at least 7 A protease component , The molecular weight ranges from 68-130KD Unequal . It can be used for isolation of pancreatic islet tissue , Separate connective tissue into individual cells .

4、 Collagenase Ⅱ For the liver , bone , thyroid , Heart and salivary gland tissue .

AI Meijie Worthington  Collagenase Use of ：

Crude collagenase is widely used in enzymatic primary cell separation and tissue dissociation procedures . Most researchers either use crude collagenase preparations , for example  1、2、3  and  4  type , Either use chromatographic purified collagenase （ Code ：CLSPA）; The latter is usually associated with secondary enzymes such as elastase 、 Hyaluronidase, etc . For best results , A more precise mixing of proteolytic activity must be adjusted for the tissue to be dissociated . There is a good correlation between the types and effectiveness of different organizations , But it's not perfect , And may depend in part on the parameters and objectives used and the differences between batches .

AI Meijie Worthington Collagenase Multi type study of （ Part of the show ）：

1、 Collagenase , purification

Chromatographic purification .≤ 50  Casein units / mg . Provided in the form of lyophilized powder .

Stored in the  2-8°C.

2、Collagenase Vial, NCIS

NCIS  A component of the kit . The material has been subjected to  0.22  Micron membrane filtration and freeze-drying in autoclave . use  5 ml HBSS  Or equivalent reconstituted vials  300  Company /ml  Collagenase solution , Code ：CLSPA. It is suitable for cell separation and culture .

Stored in the  2-8°C.

3、 Collagenase / Elastase bottle  (CLSH)

HIS  The enzyme bottle of the kit , contain  20,000  Unit collagenase （ Code ：CLS-1） and  30  Unit elastase （ Code ：ESL）, adopt  0.22  Micron membrane filtration and freeze-drying .

Stored in the  2-8°C.

4、 Collagenase ,1  type

The original balance of enzyme activity . Each batch was tested for collagenase 、 Casein enzyme 、 Clostridium protease and trypsin activity . Recommended for epithelial cells 、 Hepatocytes 、 The primary cells of lung and adrenal gland were isolated . A lyophilized powder for dialysis .

Stored in the  2-8°C.

5、 Collagenase ,2  type

Preparation contains higher Clostridium protease activity . Recommended for bones 、 The heart 、 The liver 、 Primary cell separation of thyroid and saliva . Dialysis 、 Available in the form of lyophilized powder .

Stored in the  2-8°C.

6、 Collagenase ,3  type

The secondary proteolytic pollutants have low activity , But it has typical collagenase activity . It is suggested to be used for primary cell separation of mammary gland . A lyophilized powder for dialysis .

Stored in the  2-8°C.

7、4  Type collagenase

The preparation contains a low level of trypsin activity , To limit the damage of proteins and receptors , But collagenase activity is normal or higher than normal . Recommended for primary islet isolation . A lyophilized powder for dialysis .

Stored in the  2-8°C.

8、5 Type collagenase

After partial purification , Contains higher collagenase and casein enzyme activities . A lyophilized powder for dialysis .

Stored in the  2-8°C.

9、 Collagenase ,6  type

The preparation contains higher than normal collagenase 、FALGPA  And caseinase activity , And designed to be rich in  II  type  ( col H )  Collagenase .

Stored in the  2-8°C.

10、 Collagenase ,7  type

The preparation contains more than collagenase  1-4  many  4-5  Fold collagenase 、FALGPA  And caseinase activity , It has moderate activity of Clostridium protease and trypsin .

Stored in the  2-8°C.