Chemical properties and application of trypsin

Trypsin is a kind of digestive enzyme , It is mainly secreted by the pancreas . Human pancreas can secrete trypsinogen , Trypsinogen is the premise of trypsin , Trypsinogen comes into contact with proteins in the gastrointestinal tract 、 Fatty foods or stomach acid , It is rapidly activated to form trypsin , For protein 、 Fat has the ability to digest 、 Decomposition , It can produce amino acids that can be absorbed by the human body 、 Fat emulsion, etc . therefore , Trypsin has a very important digestive function . When the bile duct or pancreatic duct is blocked , Trypsinogen is activated to damage the pancreas itself , Form acute pancreatitis .

The role of trypsin ：

The function of trypsin is to clear blood clots 、 Pus 、 Necrotic tissue and inflammatory exudate , For necrotic trauma 、 Ulcer 、 hematoma 、 Adjuvant treatment of abscess and inflammation . Ophthalmology uses this product to treat various eye inflammation , Such as hemorrhagic eye disease and eye trauma 、 Retinal concussion, etc . This product can also be used for snakebite , Break down the toxin 、 damage . Trypsin acts as an endopeptidase , It can selectively act on denatured proteins , Hydrolyze it into polypeptides or amino acids , Improve tissue permeability , Inhibit the inflammatory reaction around edema and thrombosis , Dissolve blood clots 、 Exudate and necrotic tissue , It can also decompose sputum 、 Mucinous secretions such as pus , Promote the rapid diffusion of local drug solution 、 absorption . It should be noted that , Trypsin should not be used in acute inflammatory sites 、 Bleeding cavity 、 Pulmonary hemorrhage within one week 、 Hepatorenal insufficiency 、 Patients with abnormal hemagglutination mechanism and bleeding are forbidden . All in all , As a therapeutic drug, it is recommended to use it under the guidance of a doctor .

AI Meijie Worthington Trypsin Chemical properties of ：

Chinese name ： Trypsin , Modified, SequENZ Sequencing Grade

An English name ：Trypsin, Modified, SequENZ Sequencing Grade

cat#：WBC-LS02124

specifications ：Bulk

Active site residues ：

Histidine  (H63)

Aspartic acid  (D107)

Serine  (S200)

Activator ：  Using lanthanides instead of calcium ions can improve the conversion of trypsinogen

CATH  classification  (v. 3.2.0):

Category ： Mainly  Beta

framework ： Beta barrel

topology ： Thrombin ,H  Subunits

Molecular weight ：  23.3 kDa（ Theoretical value ）

Best  pH  value ：  7.5-8.5

Isoelectric point ：

Trypsinogen ：pH 9.3

Trypsin ：pH 10.5

AI Meijie Worthington Trypsin Application ：

Tissue dissociation , Especially when combined with other enzymes such as collagenase and elastase

adopt “ Trypsin digestion ” Harvest cells

Mitochondrial separation

In vitro study of proteins

Remove monolayer cells from plastic and glass

Various hemagglutination procedures

Flow cytometry  DNA  Sample preparation for analysis

Trypsin map

Fingerprinting and sequencing

The environmental monitoring

Reduce cell density in tissue culture

Passage cells

Cleave the fusion protein

Glycopeptides are produced from purified glycoproteins