Biovendor sRAGE protein solution

sRAGE: soluble RAGE,  Soluble RAGE：

Except for the full length RAGE Outside , There are also two types of shears , One is N End shear body , Function unknown ; One is C End shear body , Lack of transmembrane region , Secreted in plasma , In soluble form , Also called esRAGE (endogenous secretory RAGE).sRAGE Able to combine AGEs Isoligand , Prevent it from contacting the full length of the membrane RAGE combination , So as to block the vascular damage caused by its signal transduction pathway , At the same time, it helps AGEs And so on . therefore sRAGE It can protect blood vessels , It can adjust in a negative direction AGEs Receptor dependent pathogenesis . in vivo RAGE There are individual differences in the level of shear body , This makes patients with diabetes have different susceptibility to vascular disease .

AI Meijie  BioVendor sRAGE protein chemical property ：

type ： Recombinant protein

resources ： E. coli

Other names ： Soluble receptors for advanced glycation end products ,sRAGE, Advanced glycation end products specific receptors ,AGER,RAGE

species ： human beings

describe ： A total of  339 AA. Molecular weight ：36.5 kDa.UniProtKB acc.no. Q15109.N  End  His-tag 14 AA（ highlight ）.

The purity ： Purity determined by optical density image analysis ：> 95%

SDS-PAGE Gel: Set & Match ：

people  sRAGE  Of  12% SDS-PAGE  Separate

1. MW  Mark – 14, 21, 31, 45, 66, 97 kDa

2.  Reduce and heat the sample ,5μg/ lane

3.  Non reducing and non heating samples ,5μg/ lane

Endotoxin ：< 0.1  The European Union / MCG

The formula ： Filter （0.4 μm） And in  0.03 M  In acetate buffer with  0.5 mg/mL  Concentration freeze-drying ,pH=4.0

restructuring ： Join in  0.1 M  Acetic acid buffer  pH4, Preparation about  0.5 mg/mL  Working stock solution , Allow the lyophilized particles to dissolve completely . In order to transform into higher  pH  value , We recommend that it be diluted sufficiently to... With the relevant buffer  10 μg/mL  The concentration of . At higher concentrations , The solubility of the antigen is limited . Before being used in cell culture , For media containing this non sterile product / The working solution is filtered and sterilized .

sRAGE protein turn Application of Science ： Western blotting 、ELISA

Storage / expire ： Store lyophilized protein in –80 °C. stay –80 °C  When storing under , Freeze dried protein remains stable before shelf life . Repack the recombinant protein to avoid repeated freezing / Thaw cycle , And in –80 °C  Under long-term storage . The recombinant protein can be used in  4°C  Next week .

Quality control testing ：

BCA  Determine the amount of protein .

SDS PAGE  To determine the purity of the protein .

LAL  To determine the amount of endotoxin .

* For research purposes only .

AI Meijie  BioVendor sRAGE protein relevant The literature ：

1、Liu F, Teodorowicz M, Wichers HJ, van Boekel MA, Hettinga KA. Generation ofSoluble Advanced Glycation End Products Receptor (sRAGE)-Binding Ligands duringExtensive Heat Treatment of Whey Protein/Lactose Mixtures Is Dependent onGlycation and Aggregation. J Agric Food Chem. 2016 Aug 24;64(33):6477-86. doi:10.1021/acs.jafc.6b02674. Epub 2016 Aug 15. PubMed PMID: 27460534. See more on PubMed

2、Zenker HE, Ewaz A, Deng Y, Savelkoul HFJ, van Neerven RJJ, De Jong NW, Wichers HJ, Hettinga KA, Teodorowicz M. Differential Effects of Dry vs. Wet Heating of β-Lactoglobulin on Formation of sRAGE Binding Ligands and sIgE Epitope Recognition. Nutrients. 2019 Jun 25;11(6):1432. doi: 10.3390/nu11061432. PMID: 31242665; PMCID: PMC6627217. See more on PubMed