Example analysis of recombinant monoclonal antibody prosci CD154 antibody

AI Meijie ProSci CD154  antibody chemical property ：

Species reactivity ： human beings

species ： The rabbit

The name of the original species ： rat

Chimeric use statement ： This chimeric rabbit antibody uses primitive humans  IgG1  Format of variable field sequence , To improve with existing reagents 、 Determine compatibility with technology .

Immunogen ： The initial monoclonal antibodies were produced by using human  gp39 (gp39-CD8)  Immune production of soluble fusion protein .

conjugate ： Unbound

The test application ：Bl, IF

ProSci CD154  Antibody case analysis ：

chart ： Use anti  CD154  antibody  IDEC-131 (Toralizum678)  To be fixed  Jurkat  The cells were stained with immunofluorescence  Shi-fix The paraformaldehyde on the cover glass is fixed  Jurkat  Immunofluorescence analysis of cells , use  10 ug/ml  Chimerism of  r  edition  IDEC-131 (Toralizum04)  dyeing  1  Hours and then  Alexa Fluor 488  second antibody  (2 ug/ml) -  Show membrane staining . Nuclear staining is  DAPI（ Blue ）. Panel from left - Right - On - Display below  24-804-DAPI- Merge channels and isotype control . The isotype control is an unknown specific antibody  (3.0), And then use  Alexa Fluor 488  Secondary antibody staining .

ProSci CD154  antibody characteristic ：

Same type ：IgGκ

buffer ： contain  0.02% Proclin 300  Of  PBS.

Storage conditions ： stay  4C  It can be saved for up to  3  Months . For longer storage time , Please repack and store  -20C.

Other names ：CD40L、gp39、TRAP、 Members of tumor necrosis factor ligand superfamily  5、TNFSF5、T  Cell antigens  Gp39、TNF  Related activator protein 、TNF  Related activator protein